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Lancaster will select his starting side and eight replacements from the same matchday 23 chosen for the 12-6 victory over Ireland in Dublin on February 10 -- a result that left England top of the table and the only side capable of winning a Grand Slam Six Nations.
Wasps No 8 Billy Vunipola had been pressing hard to force his way into the matchday line-up but his prospects suffered a setback when he injured his ankle in training on Tuesday.
As a result, Lancaster has opted for the same 23 he fielded at Lansdowne Road although that would not prevent him making changes to a starting line-up due to be announced on Thursday.
"We had to make some close calls but the team did very well in Ireland so we've gone with the same squad against France," Lancaster said Tuesday.
"We are looking forward to getting back to Twickenham against what I am sure will be a highly motivated French team."
France coach Philippe Saint-Andre has already labelled Saturday's match a "Grand Slam" for his struggling side with the pre-tournament favourites in the shock position of being bottom of the table after defeats in the opening two rounds by Italy and Wales.
No France side has lost its first two Six Nations matches, with the last time 'Les Bleus' suffered back-to-back losses at the start of the Championship coming in 1982 during the Five Nations era.
England 23-man squad to play France in a Six Nations international at Twickenham on Saturday (1700GMT kick-off):
Backs (10): Chris Ashton (Saracens), Brad Barritt (Saracens), Mike Brown (Harlequins), Danny Care (Harlequins), Owen Farrell (Saracens), Toby Flood (Leicester), Alex Goode (Saracens), Manu Tuilagi (Leicester), Billy Twelvetrees (Gloucester), Ben Youngs (Leicester).
Forwards (13): Dan Cole (Leicester), Dylan Hartley (Northampton), James Haskell (Wasps), Joe Launchbury (Wasps), Courtney Lawes (Northampton), Joe Marler (Harlequins), Geoff Parling (Leicester), Chris Robshaw (Harlequins, capt), Mako Vunipola (Saracens), David Wilson (Bath), Thomas Waldrom (Leicester), Tom Wood (Northampton), Tom Youngs (Leicester)