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Italy's health ministry on Saturday said tests carried out on Nestle beef products seized this week had shown they did not contain horse meat.
"There was no trace of equine DNA in the cooked and frozen minced beef that was seized from Nestle on February 21," the ministry said in a statement.
"The meat will therefore be returned," it added.
Nestle, the world's biggest food company, on Monday said it was withdrawing two types of pasta meal from supermarket shelves in Italy and Spain due to contamination that did not constitute a health risk.
Italian police on Thursday seized 26 tons of beef from Nestle.