Steve Mullings, a member of the Jamaican 4x100 metres team that won world athletics gold in 2009, failed to have his life ban overturned by The Court of Arbitration for Sport (CAS) on Monday.
The 30-year-old - who also won 4x100m relay silver at the 2007 world championships - had appealed against the decision handed down by the Jamaican Disciplinary Anti-Doping Commission (JADCO) in November 2011.
JADCO had been so severe because he had failed a doping test for the second time in 2011 for Furosemide, having tested positive in 2004 for methyltestoterone.
"The athlete had not presented any basis to challenge the testing procedure of the 2011 sample," read the CAS judgement.
"Furthermore, while Mullings has attempted to raise suspicion about his first violation the CAS Panel did not find that the circumstances surrounding the first offence did warrant a more lenient sanction and, accordingly, it confirmed the lifetime ban."