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Dutch prince Friso, the brother of King Willem-Alexander, died on Monday 18 months after he was left brain-damaged by an avalanche while skiing in Austria, the palace said.
"His Majesty the King (Willem-Alexander) announces with great regret that this morning his highness prince Johan Friso... died at the Huis ten Bosch Palace in The Hague, aged 44," a palace statement said.
"Prince Friso died from complications as a result of oxygen shortages during his ski accident on February 17, 2012," the statement said.
The prince had "minimal consciousness" and his condition was unchanged, the palace said.
Friso, the younger brother of Willem-Alexander, was injured while skiing off-piste in the Austrian Alps in February, 2012.
Friso was in July transferred from a hospital in London, where he lived, to the residence of his mother, former queen Beatrix, in The Hague.
In 2004, Prince Friso married Mabel Wisse Smit, giving up his claim to the throne as well as his Royal House position after it emerged that his future wife had withheld details of her previous relationship with a Dutch drug baron and the prime minister at the time declined to ask parliamentary permission.
At the time, Prince Friso was fourth in line to the throne.
The couple had two children.