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Prime Minister Benjamin Netanyahu had vowed to prevent a repeat of a similar march last month, in which hundreds of people managed to breach Israel's borders.
Israeli soldiers have opened fire on pro-Palestinian protesters as they tried to march on the heavily fortified Golan Heights, killing at least three people and wounding nine, Syrian state television reported.
The protesters were marking the 44th anniversary of the 1967 Six-Day War when Israel seized the Golan Heights from Syria.
Israel had said it would prevent a repeat of a similar march last month, in which hundreds of people managed to breach the border fence.
On May 15, Israeli gunfire killed 12 people and wounded hundreds as demonstrators marched on Israel's borders to mark the anniversary of the Jewish state's 1948 creation, known in Arabic as the "nakba" or "catastrophe."
Several hundred demonstrators on Sunday around midday marched on the razor-wire fencing near the village of Majdal Shams in the Golan Heights. Some chanted slogans and hurled rocks and garbage as they scaled the fence at several locations to try to enter the Golan, Syrian state-run TV reported.
Israeli soldiers reportedly shouted warnings in Arabic at the protesters and fired shots in the air, before shooting directly at the people. CNN reports that the Israeli forces also fired tear gas at the crowd.
At least three people, including a child, were killed, and nine others wounded Sunday by Israeli gunfire, Syrian TV reported.
A Reuters correspondent reported seeing at least four demonstrators carried away on stretchers, but it was not immediately clear if they had been hit by Israeli gunfire.
Earlier in the day, Israeli Prime Minister Benjamin Netanyahu said he had instructed security forces to act “with determination and restraint” to protect the country’s border fences, and said he would not allow "extremists" to breach Israel's borders.